For this example to working properly, You need to do following steps:
1) Install server certificate.
2) Enable SSL on IIS.
3) set secure communication to require client certificate.
4) install client certificate

Key	Value
ISSUER	<%=Request.ClientCertificate("ISSUER") %>
SUBJECT	<%=Request.ClientCertificate("SUBJECT") %>
SERIALNUMBER	<%=Request.ClientCertificate("SERIALNUMBER") %>
VALIDUNTIL	<%=Request.ClientCertificate("VALIDUNTIL") %>